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Carbon-60 Third-Party Testing

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Prevention of Brain Cell Death

Toxicity testing of Greska’s Carbon-60™ for was conducted at the Knoebel Institute for Healthy Aging (KIHA),

The University of Denver, located in Denver, Colorado.

In his continued testing, Dr. Parades was interested in taking the toxicity experiment to the next level. Given the reported antioxidant properties of Carbon 60, Dr. Paredes investigated the potential of Greska’s Carbon-60 on cell protection upon confronting the cells with an extremely oxidant environment. In the next set of experiments, the goal was to observe Greska’s Carbon-60™ reaction when hydrogen peroxide (H2O2) was added to the cell culture media to induce cell death.

Hydrogen peroxide is a potent oxidizing agent and can be used for bleaching, as a disinfectant, and even as a rocket fuel component. In the scientific research community, it is common practice that hydrogen peroxide is used to induce cell death in scientific research and experiments.

Using the same rat brain cell culture as was used in the toxicity testing, the control sample and Greska’s Carbon-60 were prepared. After the addition of the hydrogen peroxide, following standard procedure, the expectation is to see significant cell death within 24 hours. When both samples were evaluated after 24 hours, the control sample (without Greska’s Carbon-60™) showed 80% cell death. What was astounding was the brain cell culture with Greska’s Carbon-60™ added, when checked at the 24-hour point, there was only 40% cell death. That is an increase in the survival rate of 50% when compared to the non-treated cells.

According to Dr. Parades, “Adding hydrogen peroxide to this cell culture is like throwing an atomic bomb in the petri dish. There’s nothing that can prevent cell death. To see this level of survival rate is remarkable.” Dr. Parades continues, “Even though this experiment was done in rodent cells, there is a great probability that such protective effect of the Greska’s Carbon-60™ that we observed, also happens in a whole organism, including humans, this result merits further research to fully explore the potential of this molecule (Greska’s Carbon-60) in the treatment of devastating conditions where oxidative stress is at the core of cell death”

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MASS SPECTROMETRY CERTIFICATION OF ANALYSIS

Mass Spectrometry Certification of Analysis (COA) of Greska’s Carbon-60 powder confirming Carbon 60 molecules.

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Mass Spectrometry Certification of Analysis (COA) of Greska’s Carbon-60 powder confirming Carbon 60 molecules.This is the same test used to confirm Carbon 60 and award the Nobel Prize to Smalley, Kroto and Curl in 1996 for the discovery of Carbon 60.

Note the peak at 720 atomic units. The atomic unit of a single atom of carbon equals 12 atomic units. The 60 carbon atoms times 12 atomic units equals 720 atomic units. The graph spike at 720 atomic units confirms Carbon 60.

Mass Spec Time of Flight tests courtesy of Mass Spectrometry Laboratory, School of Chemical Sciences University of Illinois Urbana-Champaign.

HEAVY METAL TEST

Greska’s Carbon-60™ was tested for heavy metals at Steep Hill Laboratories, Hawaii

Greska’s Carbon-60™ was tested for heavy metals at Steep Hill Laboratories.Heavy metals Certificate of Analysis (COA) showed Greska’s Carbon-60 showed no Heavy Metals were detected in Greska’s Carbon-60 Organic Sunflower Oil to the accuracy of the detection limits.
Courtesy of Steep Hill Laboratories Hawaii.

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TRANSMISSION ELECTRON MICROSCOPE

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TEM (Transmission Electron Microscope)

Microscope pictures of Greska’s Carbon-60 powder showing the uniform size and consistent spherical shape of Greska’s Carbon-60 powder.
Transmission Electron Microscope pictures courtesy of NanoComposix, Inc

ELEMENTAL ANALYSIS

Transmission Electron Microscope Laboratory at the Colorado School of Mines, College of Material Sciences.

99.48% CARBON AND 0.52% OXYGEN (No Other Elements or Contaminants)

The HAADF (High Angle Annular Dark Field Imaging), our sample showed all carbon except for 0.52% oxygen. No other trace elements showed up in Greska’s Carbon-60 used to quantify the exact amount of each element.

These pictures show a comparison of the contaminants found in other producers of Carbon 60 powder as compared to Greska’s Carbon 60 powder. 

According to the Transmission Electron Microscope Laboratory at the Colorado School of Mines, College of Material Sciences, not one atom of another element (besides oxygen) was present as a contaminant in Greska’s Carbon 60 powder.

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shelflife
Sunflower Shelf Life

SHELF LIFE OF GRESKA’S CARBON-60™

Certification of Analysis of Sunflower oil used by Greska’s Carbon-60.

In an effort to be as transparent as possible, Greska’s Carbon-60™ is posting the technical datasheet of our shelf life study provided by Amtek.
This shows the purity of our food-grade Sunflower Oil and the efforts the manufacturer takes to ensure it is non-GMO and is Organic. This data sheet also contains nutritional information.